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spaP gene of Streptococcus mutans in dental plaque and its relationship with Early Childhood Caries
Pubblication date: 31/2011
Authors: G.L. Durán-Contreras1, Hilda H.H. Torre-Martínez1, E.I. de la Rosa2, R.M. Hernández3, M. de la Garza Ramos4
Institution: Universidad Autónoma de Nuevo León, Mexico.
1 Departament of Odontopediatrics. Dentistry School,
2 Department of Molecular Biology, Biología molecular, Chemical Science School,
3 Department of Biostatistics, Biological Science School,
4 Departament of Microbiology, Dentistry School
Publication: European Journal of Paediatic Dentistry
Publisher: Ariesdue Srl
Keywords: Dental plaque; Early Childhood Zaries; PCR; Streptococcus mutans.
Title: spaP gene of Streptococcus mutans in dental plaque and its relationship with Early Childhood Caries
Abstract: Aim Streptococcus mutans and Streptococcus sobrinus are the main pathogens associated with the development of dental caries in humans. Recently, the real-time polymerase chain reaction (qPCR-TR) has been used for fast and exact quantification of these bacteria species. This molecular biology method has made the detection of these bacteria in saliva and dental plaque possible; additionally, it aids the development of illness risk prediction. The purpose of this prospective, analytic, transversal, observational and unicenter study was to quantify the spaP gene of the Streptococcus mutans and its correlation with caries in a group of children using isolated DNA from plaque samples processed through qPCR-TR, using specific oligonucleotides for this gene detection.
Materials and methods The cariogenic potential of Streptococcus mutans in the dental plaque was analysed in a group of patients aged 12 to 46 months. A descriptive statistical analysis was performed. The Spearman’s correlation coefficient was used to establish the correlation between caries (dmft) index (decayed/missing/filled primary teeth), spaP gene and age group. The Wilcoxon test was used to compare MSB cultivation technique and qPCR-TR.
Results and conclusion In the molecular trials, a close association between caries prevalence in childhood and the presence and high proportion of the spaP gene of S. mutans was found. The average caries prevalence was 3.71, and it increased as age range increased. The highest caries prevalence was observed in female patients and in the oldest age range studied (40–46 months) which contrasts with the 12-18 months age that had a caries (dmft) index of zero. The amplification using as initiator the gene spaP of the nucleic acids extracted from the S. mutans resulted positive in 91.3% of the cases. Every child with caries was positive for the spaP and only 8.75% were negative, this group included children without caries. In conclusion, there was a correlation with infant caries prevalence and S. mutans.